Molecular Genetics

Facts

A variety of marker pairs within a few Mbp of one another on the molecular map were used as probes in the FISH protocol. The targets of probe hybridization were interphase nuclei instead of metaphase or prometaphase chromosomes usually used as targets. The distances between the fluorescent spots corresponding to the two probes were measured. The square of the distances was plotted against the molecular distance between the markers. The square of the distance was used because the statistical theory of linear polymers predicts a dependence on the square of the distance.

Interpretations

• FISH of interphase nuclei can produce fine mapping of markers that are within 2 Mbp of one another.
• Interphase FISH can order close molecular markers on the morphological chromosome.
• At the level of resolution of interphase FISH, molecular and morphological maps are colinear.
• A high resolution morphological map can help establish a molecular map of a region.

Further information

• Examples of interphase FISH are available.

This is page 1227 of Molecular Genetics by Ulrich Melcher, © 1997, 1998, 1999