Transgenic organisms can be created after transfection of stem cells.
Embryonic stem cells play important roles in medical research.
- Cells dissociated from an embryonic blastula can also be targets for transfection. Other methods may also be used to put DNA into the cells.
- Transfected cells can be returned to a blastula, and the blastula reimplanted in the female animal.
- If transfection was successful, the resulting animal is a chimera with different parts of the body coming from transfected and non-transfected cells.
- The strategy is also used to generate chimeric knock-out animals.
- Chimeric animals whose germ cells stem from successfully transfected cells can be bred and pass on the transgene to the next generation.
- The animals of this generation are homogeneously transgenic.
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This is page 436 of Molecular Genetics by Ulrich Melcher, ©1997, 1998, 1999, 2001
E-mail inquiries to U. Melcher------------Last Updated: 11 August,2001